灌注系統(tǒng)概述
HSE—HA生理學系統(tǒng)是一系列應用廣泛的完備產品，可用于整體動物生理學、立體或在體器官和組織灌注及組織浴槽研究。這些系統(tǒng)具有廣泛的應用，包括傳統(tǒng)的**藥理學、DMPK、毒理學、呼吸系統(tǒng)、心臟病學、分子生物學和生理學。每個系統(tǒng)都設計了標準的組件，再加一些其他配置以適合特殊研究的需要。。標注組件也意味著如果你是需要將現有設備配齊，或者進行系統(tǒng)升級，或者是完整的配套產品，你都只需要購買需要的構件。HSE-HA生理學系統(tǒng)為你提供各種工具，使你的研究走向多產和成功。
我們提供大多數生理學檢測設備，包括放大器、電極、傳感器和電化學檢測器。參數包括力、位移、壓力、液體和氣體流、心電圖（單導聯—12導聯系統(tǒng)）、MAP（單個或多個部位）、腦電圖、肌電圖、連續(xù)支流灌注傳統(tǒng)的血氣參數和電解質的分析，可以選擇程序化軟件和傳統(tǒng)的刺激器作為放大器、電極和傳感器等產品的補充。
HSE-DAQ系統(tǒng)產品家族（BDAS、ACAD、HAEMODYN、ISOHEART、PULMODYN、PENNOCK）可以記錄你的初始信號，計算到處的參數，注釋數據，快速簡易的輸入確定的數據用于分析。同時還可以選擇用于進一步分析的軟件模塊，帶狀記錄紙打印，自動和控制模塊等。產品經過預先設置，打開包裝即可使用，并專門設計符合你的系統(tǒng)配置和數據分析要求。
• Assessment of lung functions under cell culture conditions
• From any species (murine, rat, human)
• Study of airways of different sizes
• Allows to quantify responsiveness of simple airways and simple vessels
• Analyzing ciliary beating frequency
Lung Functions under the Microscope
Precision-cut lung slices (PCLS) offer a novel and unique way to assess lung functions under cell culture conditions. They can be prepared from nearly any species including murine, rat and human lungs. The method allows the study of the response of airways of different size (down to the terminal bronchioles) and to relate these changes in lung functions to gene expression and mediator release. Slices are viable for at least three days. They can be placed under an inverted microscope, where digital image techniques allow to quantify not only the responsiveness of single airways, but also of single vessels. In addition, it is possible to analyze the ciliary beating frequency. More than 20 slices can be obtained from one lung, thus this
method is very economical in terms of experimental costs and animal use. Tissue cores are prepared from the lungs filled with agarose solution, after cooling to
The incubation chamber was developed to allow incubation and observation of slices by an inverted microscope. The chamber is made of Polycarbonate. It is connected to a water bath to maintain constant temperature conditions. Two incubation cells are positioned in the center of the chamber. The bottom of the cells is sealed by glass, the cover is made of acrylic glass. The slices are fixed in the incubation cells by positioning them under nylon strings fixed to a bent platinum wire. The incubation cells can be filled with buffer, medium or drug solutions through the filling pipe. Buffer solution can be removed from the cells over a vacuum pipe. In addition, it is possible to gas the incubation cells in order to use bicarbonate buffered media.
The incubation chamber is placed on the stage of an inverted microscope and warmed to
Example of an Application
As an example Figure 1 shows exposure of a slice to increasing concentrations of endothelin-1. Shown is a lung slice containing an airway (B), a pulmonary artery (PA) and a pulmonary vein (PV). The pulmonary artery and the airway contracted almost completely, while the pulmonary vein area decreased to only 50% of its initial area. These responses are now easily quantified by digital imaging technique.
It is a distinct advantage of this technique that in many ways precisioncut slices can be treated like a cell culture. Thus, the slices can be incubated under various conditions and gene as well as protein expression or mediator release be determined. In contrast to cell culture models, in slices the anatomical structure of the lung is largely maintained, so that the functional consequences of gene expression and mediator release can be evaluated.
Catalog No. $ Product
BS4 73-2370Lung Slices Chamber
BS4 73-0125Thermostatic Circulator E 103, 230V AC, see page K27
Accessories
BS4 73-2802 Thermostatic Circulator E 103, 115V AC, see page K27
BS4 73-0113 Roller Pump Reglo Analogue ISM 827, 4 Channels, 0.002 to 30 ml/min